presage st2 elisa kit  (Critical Diagnostics)

Journal: Journal of Clinical Medicine

Article Title: Soluble Isoform of Suppression of Tumorigenicity 2 (ST2) Biomarker in a Large Cohort of Healthy Pediatric Population: Determination of Reference Intervals

doi: 10.3390/jcm11164693

Figure Lengend Snippet: ST2 results in ng/mL for all participants and categorized per age and sex. Mean, standard deviation, 95% confidence intervals of the mean, reference intervals and 90% CI of lower and upper limits are represented.

Article Snippet: Using the same ST2 assay (sandwich ELISA kit from Presage © ST2 assay, Critical Diagnostics, San Diego, CA, USA), the median ST2 value obtained in our pediatric cohort (14.7 ng/mL) was lower than the one reported by Meeusen et al. (i.e., 21 ng/mL) [ ], Caselli et al. (i.e., from 16.6 to 23.0 ng/mL) [ ] and Hauser et al. (i.e., 17.7 ng/mL) [ ].

Techniques: Standard Deviation

Journal: Journal of Clinical Medicine

Article Title: Soluble Isoform of Suppression of Tumorigenicity 2 (ST2) Biomarker in a Large Cohort of Healthy Pediatric Population: Determination of Reference Intervals

doi: 10.3390/jcm11164693

Figure Lengend Snippet: ST2 concentrations among 412 pediatric subjects as a function of age and sex. Trend for ST2 as a function of age is expressed for females (pink dots) and males (blue dots). Simple linear regressions are represented with continuous lines. Dotted lines correspond to the 95% CI of the linear regression.

Article Snippet: Using the same ST2 assay (sandwich ELISA kit from Presage © ST2 assay, Critical Diagnostics, San Diego, CA, USA), the median ST2 value obtained in our pediatric cohort (14.7 ng/mL) was lower than the one reported by Meeusen et al. (i.e., 21 ng/mL) [ ], Caselli et al. (i.e., from 16.6 to 23.0 ng/mL) [ ] and Hauser et al. (i.e., 17.7 ng/mL) [ ].

Techniques:

Journal: Journal of Clinical Medicine

Article Title: Soluble Isoform of Suppression of Tumorigenicity 2 (ST2) Biomarker in a Large Cohort of Healthy Pediatric Population: Determination of Reference Intervals

doi: 10.3390/jcm11164693

Figure Lengend Snippet: ST2 concentrations according to age partitions and sex. Mean and 95% CI of the mean are represented in black.

Article Snippet: Using the same ST2 assay (sandwich ELISA kit from Presage © ST2 assay, Critical Diagnostics, San Diego, CA, USA), the median ST2 value obtained in our pediatric cohort (14.7 ng/mL) was lower than the one reported by Meeusen et al. (i.e., 21 ng/mL) [ ], Caselli et al. (i.e., from 16.6 to 23.0 ng/mL) [ ] and Hauser et al. (i.e., 17.7 ng/mL) [ ].

Techniques:

Journal: Journal of Cardiovascular Development and Disease

Article Title: The Effects of Exercise Training on Cardiopulmonary Exercise Testing and Cardiac Biomarkers in Adult Patients with Hypoplastic Left Heart Syndrome and Fontan Circulation

doi: 10.3390/jcdd9060171

Figure Lengend Snippet: Comparison of cardiac biomarker values between T0 and T1. ns = not significant.

Article Snippet: ST2 (ng/mL) was measured with the ELISA kit Critical Diagnostics Presage ® ST2 (Critical Diagnostics, San Diego, CA, USA), according to the manufacturer’s instructions.

Techniques: Comparison, Biomarker Discovery

Journal: Biosensors

Article Title: Electrochemical Immunosensing of ST2: A Checkpoint Target in Cancer Diseases

doi: 10.3390/bios11060202

Figure Lengend Snippet: Preparation of the MB-based immunosensor for the determination of ST2. ( a ) Steps involved in the sandwich immunoassay; ( b ) Electrochemical transducer and reactions involved in the amperometric readout; ( c ) Example of amperometric traces recorded for control and high ST2 content samples.

Article Snippet: PRESAGE ST2 and MBL ST2 sandwich-based ELISA kits provided LODs of 1.3 ng mL –1 and 32 pg mL –1 , respectively, which are comparable to that achieved with the developed amperometric immune platform.

Techniques: Control

Journal: Biosensors

Article Title: Electrochemical Immunosensing of ST2: A Checkpoint Target in Cancer Diseases

doi: 10.3390/bios11060202

Figure Lengend Snippet: Feasibility of the immunosensor design ( a ), and effect of the working variables on the amperometric response provided by the developed immune platform ( b–i ): ( b ) different buffered medium for the preparation of ST2 standard solutions; ( c ) concentration and ( d ) incubation time of anti-ST2-CAb; ( e ) steps involved in the working protocol; ( f ) incubation time with ST2; ( g ) concentration of btn-DAb; ( h ) Strep-HRP dilution; and ( i ) incubation time of the mixture containing btn-DAb and Strep-HRP. Amperometric responses measured in the presence of 0.0 (white bars) and 1000 ( a ) or 500 ( b–i ) (grey bars) pg mL –1 ST2 and the resulting signal-to-blank ratios ((S/B), blue dots and lines).

Article Snippet: PRESAGE ST2 and MBL ST2 sandwich-based ELISA kits provided LODs of 1.3 ng mL –1 and 32 pg mL –1 , respectively, which are comparable to that achieved with the developed amperometric immune platform.

Techniques: Concentration Assay, Incubation

Journal: Biosensors

Article Title: Electrochemical Immunosensing of ST2: A Checkpoint Target in Cancer Diseases

doi: 10.3390/bios11060202

Figure Lengend Snippet: Assessed experimental variables optimized for the ST2 immunosensor development.

Article Snippet: PRESAGE ST2 and MBL ST2 sandwich-based ELISA kits provided LODs of 1.3 ng mL –1 and 32 pg mL –1 , respectively, which are comparable to that achieved with the developed amperometric immune platform.

Techniques: Incubation

Journal: Biosensors

Article Title: Electrochemical Immunosensing of ST2: A Checkpoint Target in Cancer Diseases

doi: 10.3390/bios11060202

Figure Lengend Snippet: Calibration curves constructed for the amperometric determination of ST2 with immune platforms prepared from MBs modified with ( a ) 10 µg mL –1 or ( b ) 50 µg mL –1 anti-ST2-CAb solutions. Comparative fictitious pictures of the anti-ST2-CAb loading onto the magnetic immunoconjugates are also shown.

Article Snippet: PRESAGE ST2 and MBL ST2 sandwich-based ELISA kits provided LODs of 1.3 ng mL –1 and 32 pg mL –1 , respectively, which are comparable to that achieved with the developed amperometric immune platform.

Techniques: Construct, Modification

Journal: Biosensors

Article Title: Electrochemical Immunosensing of ST2: A Checkpoint Target in Cancer Diseases

doi: 10.3390/bios11060202

Figure Lengend Snippet: Stability of anti-ST2-CAb-MBs immunoconjugates stored in filtered PBS at 4 °C after their preparation. S/B ratio values were obtained for 0.0 and 1000 pg mL –1 ST2 standards with immune platforms prepared from the stored immunoconjugates each control day. Control limits (blue lines) were set as ±3 s of the mean value obtained the first day of the study (n = 3).

Article Snippet: PRESAGE ST2 and MBL ST2 sandwich-based ELISA kits provided LODs of 1.3 ng mL –1 and 32 pg mL –1 , respectively, which are comparable to that achieved with the developed amperometric immune platform.

Techniques: Control

Journal: Biosensors

Article Title: Electrochemical Immunosensing of ST2: A Checkpoint Target in Cancer Diseases

doi: 10.3390/bios11060202

Figure Lengend Snippet: Amperometric measurements carried out with the developed immunosensor in the presence of different serum proteins: 0.0 (white bars) and 1000 pg mL –1 (green bars) ST2 standards (and the corresponding S/B ratio, blue dots) prepared in the absence or in the presence of 5.0 mg mL –1 HSA, 1.0 and 0.1 mg mL –1 human IgG (human IgG (1) and (2), respectively) and 5.0 mg mL –1 Hb.

Article Snippet: PRESAGE ST2 and MBL ST2 sandwich-based ELISA kits provided LODs of 1.3 ng mL –1 and 32 pg mL –1 , respectively, which are comparable to that achieved with the developed amperometric immune platform.

Techniques:

Journal: Biosensors

Article Title: Electrochemical Immunosensing of ST2: A Checkpoint Target in Cancer Diseases

doi: 10.3390/bios11060202

Figure Lengend Snippet: ( a ) Comparison of the calibration plots constructed with ST2 buffered standard solutions and by adding ST2 standards to a 25-times diluted plasma sample from a healthy individual. ( b ) Concentration of sST2 (in ng mL –1 ) obtained with the developed immune platform in plasma samples grouped into pools. ( c ) Examples of the real amperometric traces recorded with the immune platform for diluted plasma samples (25-times for healthy individuals and 50-times for PDAC patients) for samples 6 (healthy individual) and 9 (PDAC patient) of . ( d ) Correlation plot for the sST2 concentration values provided by the developed immune platform and the ELISA method (individual replicates performed for the determinations in the 11 plasma samples are included in the plot).

Article Snippet: PRESAGE ST2 and MBL ST2 sandwich-based ELISA kits provided LODs of 1.3 ng mL –1 and 32 pg mL –1 , respectively, which are comparable to that achieved with the developed amperometric immune platform.

Techniques: Comparison, Construct, Clinical Proteomics, Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: Biosensors

Article Title: Electrochemical Immunosensing of ST2: A Checkpoint Target in Cancer Diseases

doi: 10.3390/bios11060202

Figure Lengend Snippet: Concentration of sST2 (in ng mL –1 ) obtained from the measurements carried out with the immune platform and the ELISA method in plasma samples.

Article Snippet: PRESAGE ST2 and MBL ST2 sandwich-based ELISA kits provided LODs of 1.3 ng mL –1 and 32 pg mL –1 , respectively, which are comparable to that achieved with the developed amperometric immune platform.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Clinical Proteomics

Journal: Frontiers in Cardiovascular Medicine

Article Title: Circulating Soluble Suppression of Tumorigenicity 2 Predicts Recurrence After Radiofrequency Ablation of Persistent Atrial Fibrillation

doi: 10.3389/fcvm.2021.653312

Figure Lengend Snippet: Enhanced plasma soluble suppression of tumorigenicity 2 (sST2) was found in persistent atrial fibrillation (AF) patients. Plasma levels of sST2 were measured by enzyme-linked immunosorbant assay (ELISA) kit and presented by dot plots. PaAF, paroxysmal AF; PeAF, persistent AF. * P < 0.01 vs. PeAF.

Article Snippet: Levels of sST2 were measured by using human sST2 enzyme-linked immunosorbant assay (ELISA) kit (Presage ST2 Assay, Critical Diagnostics, USA).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Cardiovascular Medicine

Article Title: Prognostic Value of Circulating sST2 for the Prediction of Mortality in Patients With Cardiac Light-Chain Amyloidosis

doi: 10.3389/fcvm.2020.597472

Figure Lengend Snippet: A new scoring system for predicting overall survival incorporating sST2, troponin I, NT-proBNP, and dFLC. A new scoring system including ST2 and other known cardiac risk factors, such as the dFLC and Mayo 2012 staging systems, was built to predict overall survival. We assigned 1 point for sST2 ≥12.34 ng/ml, troponin I >0.025 μg/L, NT-proBNP >1,800 pg/ml, and dFLC >180 mg/L, as reported before. The maximum score was 4, and the minimum score was 0. Score 0, none of the four conditions are met; Score 1, one condition is met; Score 2, two conditions are met; Score 3, three conditions are met; Score 4, all four conditions are met. There were 0 (2), 1 (9), 2 (19), 6 (17), and 6 (9) death events in the 0–4 scoring system, respectively.

Article Snippet: The serum levels of sST2 were measured using a Presage ST2 sandwich ELISA kit (Presage® ST2; Critical Diagnostics, San Diego, CA, USA).

Techniques: